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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Estrogen Impairs Adipose Tissue Expansion and Cardiometabolic Profile in Obese-Diabetic Female Rats
doi: 10.3390/ijms222413573
Figure Lengend Snippet: Ovariectomy and E2 treatment effects on oral glucose and insulin tolerance tests in female ZDF rats. ( A ) Oral Glucose Tolerance Test (OGTT). ( B ) Insulin Tolerant Test (ITT). In A and B, left panels represent blood glucose curves and right panels represent AUC. Blood samples were obtained from the tail vein at indicated time points. ( C ) Ratio of pAKT/AKT and protein levels of GLUT4 in WAT. ( D ) Cd36 and Ap2 mRNA expression levels in WAT. AUC, area under the curve; AKT, protein kinase B; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GLUT4, glucose transporter type 4; Cd36 , cluster of differentiation 36; Ap2 , adaptor protein 2. In C pAKT protein bands were normalized to total AKT protein intensity, and GLUT4 protein bands were normalized to GAPDH loading control. mRNA levels were normalized to housekeeping Gapdh expression. In C and D, data are expressed in arbitrary units (au) related to LEAN group. Values are expressed as the mean ± SEM. Differences between obese-diabetic (SHAM) and control (LEAN) animals were analyzed by Student’s t -test. Differences between obese-diabetic groups with different hormonal manipulation were analyzed by One-way ANOVA with Fisher’s LSD post-hoc test. Student’s t -test: a, SHAM vs. LEAN. One-way ANOVA: b, OVA or OVA + E2 vs. SHAM; c, OVA + E2 vs. OVA.
Article Snippet: Antibody for adiponectin (26 kDa, 3553) was supplied by
Techniques: Expressing